Thermostable beta-glucosidase b
Webb28 sep. 2001 · A β-glucosidase gene (bglB) of an extremely thermophilic eubacterium, Thermotoga maritima was expressed in Esherichia coli to yield the active enzyme. The … WebbThermostable beta-glucosidase BBETA-MERCAPTOETHANOLCHLORIDE IONGLYCEROL NCBI National Center forBiotechnology Information Search 7MS2: Three-dimensional …
Thermostable beta-glucosidase b
Did you know?
Webb1 apr. 1993 · The native beta-glucosidase has a molecular mass of 230 +/- 20 kDa, composed of 58 +/- 2-kDa subunits. The enzyme has a pI of 4.40. Thiol groups are not … Webb14 apr. 2024 · Abstract Lignocellulosic biomass has emerged as a promising environmental resource. Enzyme catalysis, as one of the most environmentally friendly and efficient tools among various treatments, is used for the conversion of biomass into chemicals and fuels. Cellulase is a complex enzyme composed of β-glucosidase (BGL), …
Webb28 dec. 2024 · This study mainly aims to find the optimal conditions for immobilizing a non-commercial β-glucosidase from Aspergillus niger via cross-linked enzyme aggregates (CLEAs) by investigating the effect of cross-linking agent (glutaraldehyde) concentration and soy protein isolate/enzyme ratio (or spacer/enzyme ratio) on the … WebbA gene and a truncated open reading frame orf1 encoding respectively a beta-glycosidase ... > beta1-2 (71%) > beta1-4 (40%) > beta1-6 (10%). Ttbeta-gly is a thermostable enzyme displaying an optimum temperature of 88 degrees C and a half life of 10 min at 90 degrees C. ... beta-Glucosidase / genetics* Substances
Webb26 okt. 2015 · The enzyme was stable over broad pH range of 4.0–7.0 with relative residual activity above 60% after 180 min of incubation. β -glucosidase demonstrated high thermostability with 83% of its original activity retained at 70°C after 180 min of incubation. Webb1 nov. 2024 · Request PDF Cloning, biochemical characterization and molecular docking of novel thermostable β-glucosidase BglA9 from Anoxybacillus Ayderensis A9 and its application in de-glycosylation of ...
Webb10 dec. 2012 · Biochemical characterization and mechanism of action of a thermostable beta-glucosidase purified from Thermoascus aurantiacus. Biochemical Journal, 353 …
WebbThe gene encoding a thermostable beta-glucosidase (cel3a) was isolated from the thermophilic fungus Talalaromyces emersonii by degenerate PCR and expressed in the filamentous fungus Trichoderma reesei. The cel3a gene encodes an 857 amino acid long protein with a calculated molecular weight of 90.59 … mark richman propertiesWebb27 dec. 2024 · At pH 7.0 and 40°C, Bgy3 selectively removed the outer sugars of C-20 and C-3, and it produced ginsenoside F2 from ginsenoside Rb1, with a corresponding molar conversion yield of 45%. Bgy3 was found to hydrolyze the ginsenoside Rb1 according to the following pathways: Rb1 → gypenoside XVII → F2, or Rb1 → Rd → F2. navy girls soccer id campWebb21 maj 2009 · The thermostability of β -glycosidase from S. solfataricus is determined by a network of ion pairs, located on the tetrameric surface, which results in destabilization of the enzyme under the basic conditions (D’Auria … mark richman esqWebb1 jan. 1995 · Thermostable beta-glucosidases In book: Enzymatic Degradation of Insoluble Carbohydrates (pp.197-207) Publisher: American Chemical Society, Washington, DC Editors: J. N. Saddler, M. H. Penner... mark richmond petoskey miWebb15 feb. 2012 · Of the reported β-glucosidases, P. furiosusβ-glucosidase exhibited the highest thermostability, k (cat), k (cat)/K (m), yield, and productivity for hydrolyzing genistin. These results suggest... navy girls school coatWebbThe selectivity of alcoholysis compared to hydrolysis was higher than for other reported enzymes. Both beta-glucosidases were also tested in reactions cleaving off glucose … mark richmond age singaporenavy girls school shoes