Phosphorylation of irf3 antibody

Author: fgoz

August undefined, 2024

WebSep 25, 2015 · Viral infection activates the transcription factors NF-κB and IRF3, which contribute to the induction of type I interferons (IFNs) and cellular antiviral responses. Protein kinases play a critical role in various signaling pathways by … WebJan 4, 2006 · The protein kinase TBK1 is a key regulator inducing phosphorylation of IRF3. The regulatory mechanisms during IRF3 activation remain poorly characterized. In the present study, we have identified by yeast two-hybrid approach a specific interaction between IRF3 and chaperone heat-shock protein of 90 kDa (Hsp90).

STAT2 hinders STING intracellular trafficking and reshapes its ...

WebMar 23, 2001 · Although the broad-spectrum kinase inhibitor staurosporine potently blocked IRF3 and -7 phosphorylation, inhibitors for protein kinase C, protein kinase A, MEK, SAPK, … WebWestern blot analysis of Phospho-IRF3 pSer385 using Phospho-IRF3 pSer385 polyclonal antibody (Product # PA5-36775) at a dilution of 1:500. Lane 1: Hela cell lysate treated with PMA (100nM, 15min), Lane 2: … how many deaf people in the world

Phosphorylation of innate immune adaptor proteins MAVS, STING, and …

WebWith chromatin immunoprecipitation assays, we have now investigated whether histone methylation and phosphorylation are also involved in transcriptional regulation by thyroid hormone receptor (TR). We found that repression by unliganded TR is associated with a substantial increase in methylation of H3 lysine 9 (H3-K9) and a decrease in ... WebPrimary Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859) Protein Recombinant Human IRF3 protein (ab205216) View more associated products Overview Product name Anti-IRF3 antibody … WebOverview Product name Anti-IRF3 (phospho S396) antibody See all IRF3 primary antibodies Description Rabbit polyclonal to IRF3 (phospho S396) Host species Rabbit Tested … high tech high school in lake balboa

Transcription-independent regulation of STING activation and

Phospho-IRF3 (Ser386) Polyclonal Antibody - Thermo Fisher Scientific

WebUse Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3. Anti-phospho … WebThe induction of phosphorylation of STING on S366 of IRF3 on S396 was inhibited in T404E compared to WT or ... T404A, or T404E STAT2 were treated with cisplatin for 24 h. The … how many deaf people in usWeb该IRF3 Polyclonal Antibody. This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha … high tech high school jersey city nj

"WebPhosphorylated S366 is a docking site for IRF3 and is essential for the subsequent induction of Interferons (IFNs) ( 7, 8 ). Our previous work demonstrated that IRF3 binds to phosphorylated STING in the late endosomal compartment, where TBK1 phosphorylates and activates IRF3 ( 9 ). Additional proteins regulate the trafficking of STING. " - Phosphorylation of irf3 antibody

Phosphorylation of irf3 antibody

The TAK1-JNK cascade is required for IRF3 function in the innate immune

WebMar 1, 2024 · The innate sensor ZBP1-IRF3 axis regulates cell proliferation in multiple myeloma - PMC Back to Top Skip to main content An official website of the United States government Here's how you know The .gov means it’s official. Federal government websites often end in .gov or .mil. WebJan 24, 2016 · The IRF3 transcription factor is critical for the first line of defense against pathogens mainly through interferon β and antiviral gene expression. A detailed analysis …

Did you know?

WebPhospho-IRF3 (Ser396) Antibody (720012) in ICC/IF. Immunofluorescence was performed on fixed and permeabilized HeLa cells treated with Calyculin A (50 ng/mL/20 min) for … WebJan 29, 2015 · Phosphorylated MAVS and STING then bind to a positively charged surface of interferon regulatory factor 3 (IRF3) and thereby recruit IRF3 for its phosphorylation and activation by TBK1. We...

WebSep 1, 2024 · Although phosphorylation of Ser82 phenocopies that of Ser339 and Ser97 in IFN-β activation, it controls the promoter binding ability of IRF3, an activation step … WebMAP3K8 mediates the phosphorylation and repression of IRF3 homodimers to promote greater transcriptional activity through utilization ... More The induction of the intrinsic antiviral defense in mammals relies on the accumulation of foreign genetic material.

WebPhospho-IRF-3 (Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396. This antibody does not cross-react with other family members at physiological conditions. Species Reactivity: Human. Species predicted to react based on … Highly specific and rigorously validated in-house, Phospho-IRF-3 (Ser396) (4D4G) … WebD, IRF3 phosphorylation is inhibited by E3L. 293T cells were transfected with 10 μg of an IRF3 expression vector and 10 μg of E3L (lanes 3 and4) or K3L (lanes 5 and 6), infected …

WebKey features and details Rabbit polyclonal to IRF3 (phospho S396) Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Primary Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859) Protein Recombinant Human IRF3 protein (ab205216) Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

WebSep 1, 2024 · Published findings and our results show that IRF3 is a transcription factor under the tight control by phosphorylation-dependent post-translational modification. … how many deaf schools are in germanyWebIt is now well established that TBK1 is responsible for IRF3 phosphorylation in response to stimulation of several intracellular receptors that induce type-I interferons, including TLR3, … high tech high school majorsWebMar 5, 2024 · Rnf39 deficiency and knockdown both enhanced RNA virus–induced IRF3 phosphorylation (Fig. 2, A to C, and fig. S3D). Furthermore, ... The cell lysates were immunoprecipitated with Flag antibody and then immunoblotted with the indicated antibodies. The specific bands were marked by asterisk. All experiments were repeated at … how many deadpool comics are thereWebTBK1-dependent phosphorylation of IRF3 leads to its nuclear translocation and subsequent activation of transcriptional programs that ultimately lead to the production of inflammatory cytokines, ... Staining with a dsDNA-specific antibody showed that such treatment is sufficient to induce cytosolic accumulation of dsDNA (Fig 1J, ... high tech high school night coursesWebControl of IRF-3 activation by phosphorylation Interferon (IFN) regulatory factor-3 (IRF-3) is a unique member of the IRF family. Its transcriptional activity is regulated solely by … high tech high school in secaucusWebMar 21, 2024 · IRF3 (Interferon Regulatory Factor 3) is a Protein Coding gene. Diseases associated with IRF3 include Encephalopathy, Acute, Infection-Induced 7 and … high tech high school californiaWebPhosphorylation of IRF3 occurs at a cluster of serine and threonine residues in the carboxy-terminus between residues 385 and 405. This leads to an association with the p300/CBP coactivator and stimulation of … high tech high school new jersey