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Phis2载体图谱

Webb质粒名称:pHis2. 出品公司:Clontech . 质粒类型: 酵母单杂交系统. 克隆方法: 多克隆位点,限制性内切酶. 载体大小: 7207bp . 原核抗性: Kanamycin (卡那霉素) 真核抗 … http://www.kelei-biology.com/plus/view.php?aid=1071

pHis2酵母单杂交载体 631903报价/价格pHis2酵母单杂交载体质粒 …

Webb8 juli 2024 · 该载体包含以下元素: 1. 酵母 GAL1 启动子,能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白,同时能够 被葡萄糖抑制表达 2.多克隆位点可以使用的很多 … Webb2.制备感受态并将pHis2-DNA转化进入感受态细胞; 3.SD/-Trp平板检测转化结果。 二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板,3-AT的浓度一般设定在50-100mM … dalby qld population 2021 https://reneevaughn.com

酵母杂交实验常见问题——单杂知识及自激活检测篇_元件_-bait_作用

http://www.youbio.cn/product/vt1637 WebbDescription pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for … Webb武汉淼灵生物科技有限公司发布在丁香通的pHIS2质粒载体图谱序列信息Wuhan Miaoling Bioscience & Technology Co.,Ltd报价、型号、品牌等供应信息介绍,丁香通致力为 … dalby qld population 2020

PT3705-5 (PR32049) pHIS2

Category:pHIS2图谱_百度文库

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Phis2载体图谱

pHIS2质粒载体图谱序列信息_价格-厂家-供应商_武汉淼灵生物科技 …

http://www.bioon.com.cn/reagent/show_product.asp?id=3900880 WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog #K1617-1 MCS (1–41) Sac I (17) Kpn I (775) P CEN6/ARS4 minHIS3 HIS3 3' UTR & T Kanr pHIS2 7.2 kb minHIS3 …

Phis2载体图谱

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http://www.biovector.net/product/319746.html WebbThe concentration of 3-AT needed to fully suppress leaky HIS3 expression must be determined empirically for each DNA target element.pHIS2 can be maintained in both …

WebbpHis2载体价格650元,pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Kanamycin,质粒大小为7207bp。pHis2是酵母单杂交载体。 http://www.biofeng.com/zaiti/jiaomu/pHis2.html

WebbTo use pHIS2 in a one-hybrid assay, clone one or more copies of a cis-acting DNA target sequence into the MCS. Then introduce the plasmid into competent yeast cells using the … Webb6 sep. 2024 · 1)准备质粒:构建pHis2-bait质粒→提取质粒; 2)制备Y187感受态细胞:YPDA平板活化→YPDA液体扩繁→制备酵母感受态细胞;或者直接购买Y187感受态细 …

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Webb19 nov. 2012 · 是选取atg上游2000的片段,然后整个连到phis2载体上吗? 我之前做了直接取ATG上游1.5k构建到phis2上,感觉不合适。 最好还是找到自己转录因子的结合motif … biot medicationhttp://biovector.net/product/861.html biot medicalWebb质粒简介:. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … bio toastbrotWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … biot medical termhttp://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html dalby racecourseWebbVector Database. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the … dalby race clubdalby qld attractions