WebFeb 2, 2024 · An arabinose inducible system (pHERD20T) 63 was chosen for the expression of genes required for the production of antigen-displaying PHA MCL inclusions in P. aeruginosa. WebpHERD20T pUCP20T Plac replaced by 1.3-kb AflII-EcoRI fragment of araC-PBAD cassette (Qiu et al. 2008a) pHERD20T-algW algW (PA4446) from PAO1 in pHERD20T; EcoRI/HindIII This study pHERD20T-algW I239F algW (PA4446) from PAO579 in pHERD20T; EcoRI/HindIII This study pHERD20T-pilA pilA (PA4525) from PAO1 in pHERD20T; EcoRI/HindIII This study
Remodeling of O Antigen in Mucoid Pseudomonas aeruginosa via ...
WebMar 10, 2024 · NcoI and HindIII sites of pHERD20T and introduced into BW25113 F’ via chemical transformation. Liquid culture phage infections Overnight cultures of P. aeruginosa were diluted 1:100 in LB supplemented with 10 mM MgSO4, 1 mM IPTG, 0.3% arabinose and 50 μg/ml gentamicin. 90 μl of diluted bacteria were then infected with 10 μl plus size big shirt
Over-expression of SspA induces mucoidy in CF149. The
WebDec 12, 2024 · To explore the role of Pf4r in Pf4 immunity, we used Pf4 phages to infect the ΔPf4 strain in the absence of pf4r (carrying pHERD20T) and in the presence of pf4r (carrying pHERD20T‐pf4r). As expected, the ability of Pf4 to infect the ΔPf4 strain decreased 10 6 ‐fold when Pf4r was overproduced via pHERD20T‐pf4r (Fig. (Fig.5C). 5 C). WebSep 5, 2024 · A recombination cassette consisting of genes 34 to 38 of phage JBD30 (anti-CRISPR locus with large flanking regions) in plasmid pHERD20T was previously generated … WebDec 6, 2024 · pHERD20T was used as vector for the construction of all plasmids in this study (Qiu et al. 2008 ). The plasmid with the full-length PaPilA and different lengths of C-terminal truncated PaPilA was named as pPa, pPa61, and pPa82. PAO1 genomic DNA was used as template. principle of induction solver