Peasy basic seamless cloning and assembly kit

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August undefined, 2024

WebThe GeneArt™ Seamless Cloning and Assembly Kit enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any … WebMar 24, 2024 · Based on our transcriptome data, we cloned five type-B LlRR s ( LlRR1, LlRR2, LlRR10, LlRR11 and LlRR12) via RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) and performed phylogenetic analysis, subcellular localization experiments and expression pattern analysis.

Team:QHFZ-China/Notebook - 2024.igem.org

WebThermo Fisher peasy uni seamless cloning assembly kit Peasy Uni Seamless Cloning Assembly Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: … WebpEASY® -Basic Seamless Cloning and Assembly Kit 目录号： CU201-02 单价：￥620 规格： 20 rxns 60 rxns 数量： - + 说明书质检报告立即下单加入购物车产品详情介绍本产品 … k3fk オムロン

GeneArt™ Seamless Cloning and Assembly Kit - Thermo …

WebDec 1, 2024 · Recently a commercially available pEASY-Uni Seamless Cloning and Assembly Kit (TransGen Biotech) has been made based on the technology of Gibson Assembly. In … WebDec 1, 2024 · Recently a commercially available pEASY -Uni Seamless Cloning and Assembly Kit (TransGen Biotech) has been made based on the technology of Gibson … WebFast accurate construct design for all major molecular cloning techniques; Validate sequenced constructs using powerful alignment tools; Customize plasmid maps with … advortatin

AsOBP1 is required for host seeking in the malaria vector …

WebTransGen Biotech pEASY® -Uni Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional … WebCloning Vectors 1 Selected products (0/20) Contact supplier Click to select products that you want to inquire about before clicking the Contact supplier button. pEASY-Blunt3 Cloning Kit $92.30 / piece pEASY-T1 Cloning Kit $80.00 - $90.00 / set 1 set (Min. Order) pEASY-T1 Simple Cloning Kit $80.00 - $90.00 / set 1 set (Min. Order) k3es ドライバーWebThe GeneArt Seamless Cloning and Assembly Kit eliminates multiple rounds of restriction enzyme analysis and digestion, DNA end-repair, de-phosphorylation, ligation, enzyme … k3 dahon カスタム

"WebMar 28, 2024 · PDF Hemsleya chinensis is a Chinese traditional medicinal plant, containing cucurbitacin IIa (CuIIa) and cucurbitacin IIb (CuIIb), both of which have... Find, read and cite all the research ... " - Peasy basic seamless cloning and assembly kit

Peasy basic seamless cloning and assembly kit

Evaluation of pEASY-Uni Seamless Cloning and Assembly Kit to clone

WebLinearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Basic Cloning Vectors More Plasmid Sets Home Plasmids Basic Cloning VectorspUC19L Show Static … WebJul 1, 2024 · Then, the Puro-Cas9 plasmid were digested with PmeI (New England Biolabs), gel purified and recombined with these two fragments, using the pEASY®-Basic Seamless Cloning and Assembly Kit (TransGen), thus the “tggggc” at the end of HA-L were removed.

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Web5 rows · Product Details. This kit takes advantage of proprietary recombinases and homologous recombination ... WebFeatures of the pEASY-Uni Seamless Cloning and Assembly Kit Fast assembly and fast cloning: 15 minutes Easy: regardless of restriction enzyme sites of inserts, and …

WebJul 16, 2024 · We are proud to announce the launching of pEASY®-Basic Seamless Cloning and Assembly Kit! This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-5 fragments. WebJul 13, 2024 · The fused fragments were inserted into the pRB1028 at the restriction sites Hand III and Bam HI by using the pEASY ®-Basic Seamless Cloning and Assembly Kit (TransGen Biotech, Beijing, China). pRB-secYEG was constructed successfully after DNA sequencing and was electro-transferred into B. thuringiensis BMB171. The positive …

WebpEASY ®-Basic Seamless Cloning and Assembly Kit Cloning Competent Cells Trans 10 Chemically Competent Cell Trans 5α Chemically Competent Cell Trans 109 Chemically Competent Cell Trans 110 Chemically Competent Cell Trans 1-Blue Chemically Competent Cell Trans 2-Blue Chemically Competent Cell Trans 1-T1 Phage Resistant Chemically … WebPART 1: Protocols （1）Molecular cloning：【1】PCR Primers were designed by snapgene sotware and synthesized at GENEWIZ Company. PCR was performed by following the manuals of PrimeSTAR Max Premix (2X) (Taraka, R045) or 2 X EasyTaq PCR SuperMix (+ dye) (Transgene, M256).

Web5 rows · Sep 12, 2024 · Features of the pEASY-Basic CU201. Fast assembly and fast cloning: 15 minutes. Easy: ...

WebApr 12, 2024 · The subsequent ligation reaction was carried out at 50 °C according to the pEASY-Basic Seamless Cloning and Assembly Kit (TransGen Biotech), after which ligation products were transformed into E. coli DH5α. Positive colonies were screened and plasmid was isolated and sent to Sangon (Shanghai, China) for DNA sequencing. Protein purification adv papillon unionWebSeamless cloning was performed using pEASY-Basic Seamless Cloning and Assembly Kit (Transgen). Assembly of recombinant vectors Plasmid pI1818 was constructed for surface display in BCG and ZWY2 under the control of the strong mycobacterium hsp60 promoter followed by an N-terminal truncated Rv1818’ , a transportation protein anchored in the ... k3d.co.jp きたむらWebGibson was performed by following the manual of pEASY- Basic Seamless Cloning and Assembly Kit (Transgene, CU201). 【4】Endonuclease digestion and ligation The endonucleases and T4 ligase were bought from NEB Company and the experiments were performed by following instructions. 【5】Transformation k3&co ローファー