Ntf2 mediates nuclear import of ran
Web31 dec. 1998 · The nuclear import of Ran is mediated by NTF2. (a) Digitonin-permeabilized BHK21 cells were incubated for 30 min at 25°C with buffer containing an energy … Web11 nov. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2-RanGDP complex to the nuclear side of the NPCs. A direct …
Ntf2 mediates nuclear import of ran
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WebFIG. 6. Karyopherin a (Kap60) releases karyopherin b (Kap95) from RanGTP. A, Ran that was ;40% GTP and ;60% GDP bound was immobilized on a CM5 sensor chip as described under “Experimental Procedures.” Injection of 100 mg/ml Kap95 resulted in binding of 500 RU to the Ran surface. B, Ran was immobilized as in A, and 90 RU of Kap95 were … Webscientific article
WebRan is a small G protein that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein has also been implicated in the control of … WebRibbeck K, etal., EMBO J. 1998 Nov 16;17(22):6587-98. Select categories you would like to watch. Updates to this gene will be send to {{ username }}
WebRibbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D (1998) NTF2 mediates nuclear import of Ran. EMBO J 17, 6587–6598. CrossRef PubMed CAS Google Scholar Ribbeck K, Kutay U, Paraskeva E, Görlich D (1999) The translocation of transportin-cargo complexes through nuclear pores is independent of both Ran and energy. Web1 nov. 1998 · NTF2 binds RanGDP initially to NPCs and probably also mediates translocation of the NTF2-RanGDP complex to the nuclear side of the NPCs. A direct …
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Web16 apr. 1999 · NTF2 mediates nuclear import of Ran NTF2 mediates nuclear import of Ran Ribbeck, Katharina; Lipowsky, Gerd; Kent, Helen M.; Stewart, Murray; Görlich, Dirk … canara bank bommasandra ifsc codeWeb18 jan. 2002 · We also used a mutant species [Ran(T24N)], which is defective in binding to nuclear transport factor 2 (NTF2), the Ran import carrier (4, 13, 14). The rate of nuclear accumulation of FL-Ran(T24N) was <4% that of wild-type FL-Ran (Fig. 1B) and was similar to the rate of diffusion of green fluorescent protein (GFP) into the nucleus (15,16). canara bank balurghat branch ifsc codeWebtemperature-sensitive NTF2 mutants are defective in nuclear protein import. Moreover, temperature sensitive mutants of the yeast Ran homolog Gsplp can be suppressed by overexpression of NTF2, sug gesting that Ran-NTF2 interaction is required for effective nuclear protein import in vivo (Wong et al., 1997). Whether NTF2 is also required for … canara bank budge budge branch ifsc codeWeb29 okt. 1999 · Nuclear transport factor 2 (NTF2) is a small, homodimeric protein that binds to both RanGDP and xFxFG repeat-containing nucleoporins, such as yeast Nsp1p and … canara bank atm centre in cth road chennaiWebIsothermal titration calorimetry revealed that NTF2 prevents sumoylation by reducing RanGDP's affinity to RanBP2's RBDs to undetectable levels. Taken together, our findings indicate that RanGDP and not RanGTP is the physiological target for the RanBP2 SUMO E3 ligase complex. canara bank branches in maharashtraWeb7 feb. 2008 · These observations may provide an explanation how CapG nuclear import can occur when only Ran or NTF2 is added. Discussion. Nuclear accumulation of CapG in permeabilized cells does not result from passive diffusion because CapG import is inhibited when Nups are obstructed by WGA, mAb414 or importin β (45–485). canara bank branch codesWeb8 dec. 2024 · Binding of Ran to NTF2 is required for NTF2 to inhibit nuclear expansion and import of large cargo molecules. Nucleocytoplasmic translocation of NTF2 is regulated in mammalian cells. Data suggest actin accumulation in senescent cells is due to the failure of RanGTP restoration with ATP deficiency and NTF2 accumulation, resulting in decreased … fish filter too powerful