High-throughput screens for eef-2 kinase
WebSep 18, 2024 · A high-throughput screen (kinase reaction performed at room temperature) revealed the compound TCMDS-135051 as a highly selective and specific Pf CLK3 kinase inhibitor. This was confirmed in vivo by the finding that parasite strains that developed resistance against TCMDS-135051 acquired mutations in Pf CLK3. WebTwo high-throughput assays are described for eEF-2K using recombinant, tag-free enzyme purified from bacteria. The first is a fluorescence-based assay that uses the phosphorylation of a Sox-based peptide substrate by eEF-2K, which results in a 5-fold increase in fluorescence emission, allowing for continuous monitoring of the kinase activity.
High-throughput screens for eef-2 kinase
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WebSep 27, 2013 · This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our … WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput assay systems may expedite the process. Two high-throughput assays are described for eEF-2K using recombinant, tag-free enzyme purified from bacteria.
WebIntroduced, optimized and conducted an in vitro high-throughput drug screen assay for compounds with activity against liver stage malaria. Discovered and confirmed several compounds active against ... WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of …
WebThese yeast-based methods range from HTP screens to identify novel hits against promising parasite kinase targets to the identification of potential antiparasitic kinase inhibitors extracted from databases of yeast chemical genetic screens. Keywords: Drug discovery; Drug screening; High-throughput; Kinase inhibitors; Neglected diseases; Yeast. WebMay 20, 2024 · Background: Eukaryotic elongation factor 2 kinase (eEF2K) regulates the elongation stage of protein synthesis by phosphorylating eEF2, a process related to various diseases including cancer and cardiovascular and neurodegenerative diseases. In this study, we describe the identification of novel eEF2K inhibitors using high-throughput screening …
WebMay 1, 2014 · eEF-2 kinase dictates cross-talk between autophagy and apoptosis induced by Akt Inhibition, thereby modulating cytotoxicity of novel Akt inhibitor MK-2206. ... High-throughput screens for eEF-2 kinase. J Biomol Screen, 19 (2013), pp. 445-452. Google Scholar. Diggle et al., 1998.
WebApplication of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening Authors: Clint D. … signal school teamsWebeEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput … signal school microsoft teamsWebJan 24, 2014 · Using this high-throughput assay, we screened more than 7000 compounds and discovered additional small molecules that inhibit the ATM kinase and further validated these hits by secondary assays. Introduction signal schedulingWebHere, we describe the purification of the protein kinase, eEF-2K, and the utilization of three biochemical assays in the course of identifying small molecules that block its enzymatic … signals army jobsWebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of … the prodigal women by nancy haleWebAbstract. eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of … the prodigal\u0027s perspectiveWebeEF-2 kinase is a highly conserved protein kinase in the calmodulin -mediated signaling pathway that links multiple up-stream signals to the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 ( EEF2) and thus inhibits the EEF2 function. [6] [8] Activation [ edit] the prodigal you love